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High Resolution Accurate Mass Spectrometer System (For proteomics and metabolomics)


Make: Thermo Fisher Scientific

Model: Orbitrap Eclipse Tribrid Mass Spectrometer with Nano LC and UHPLC

It will be useful in:

  • Identification of protein markers of cancer cells.
  • Food Safety and Adulteration pesticides and antibiotics studies.
  • Pharmacokinetic studies for Identification of target compounds in blood plasma in animals for pre- clinical and in human for phase-I and Phase-II clinical Studies.
  • Clinical Studies in-born errors of metabolism (IEM), New born disorders (NBS), Endocrinological disorders, Therapeutic drug monitoring (TDM).
  • Forensic Studies, Toxicology and designer drugs Identification
  • Comparing the structures of a protein obtained from different sources.
  • Studying the conformational stability of a protein under stress (Thermal, pH, denaturants etc.)

Required Samples :-

Guidelines for Sample Preparation

For Proteomics

  • Prevent or minimize contamination of your sample. Contaminants can be keratins, plasticizers, polymers, detergents, and non-volatile buffers.
  • Fungal contamination in protein gels is very common, avoid such types of gels for processing.

Protein storage recommendations – Proteins in coomassie-stained gels are relatively stable at 4? or -20?. Silver-stained protein bands must be analysed as soon as possible after it is isolated and purified or should be kept frozen (i.e. -20? or -80?).

Protein solution form - must be stored and transported at -20? or -80?.

For In-gel analysis- Coomassie stain are preferred and will generally result in the best peptide recoveries and protein ID results.

  • Cut the band out as close as possible; be very selective and cut out only the centre of the band; don’t include any excess gel material.
  • Purity of the protein sample is critical for a successful analysis of the intact molecular weight and identification of a protein.

For In-solution analysis-

  • For In solution analysis the amount of protein must be 100- 150 μg for each sample.
  • It is critical that the sample does not contain any detergents, Surfactants, or polymers (even at trace levels).
  • Avoid using Triton, Tween, SDS, and PEG, similar to that during any part of the protein preparation.
  • Use HPLC/FPLC to purify and collect samples whenever possible.
  • For more than 10 samples new requisition form needs to be submitted.

General Instruction:

  • Sample will not be analysed till the payment confirmation.
  • Before sample preparation, it is advisable to contact the facility and discuss the protocol.

 

Disclaimer: The experimental data provided is only for research & development purposes. These cannot be used as a certificate in legal disputes.

 

For Metabolomics

  • The metabolites should be extracted by the user itself in the proper solvent of good quality (LC-MS or HPLC grade).
  • The sample should be pure and dried and submitted in a properly labeled vial
  • Mention the suitable solvent (preferably protonated solvent like MeOH, ACN, or Water.)

 

Note:

  • The users are requested to contact the facility in case of queries regarding the price and analysis to be used. Email of the facility: sathiservices@bhu.ac.in.
  • Sample list in Excel sheet must be provided to the facility with the weight of samples and the sample tubes must have similar codes for ease of handling (Lab name- day and month- sample number. Eg. JV-2611-01).
  • All the samples and standards must be prepared by using MS grade solvents for a good quality result.
  • Average processing time after sample submission is 3 weeks for metabolites and 4-8 weeks for proteomics. The sample processing time starts after the submitted samples.
  • After analysis the raw data will be provided without any statistical processing.
  • Facility will not be responsible for data processing/experimental results.
  • Kindly acknowledge the facility in your publications.

**Please make available the analysis related publications to expedite the sample preparation related protocols.


Form Application :-

Click to Book Online

User Charges :-

  User Charges
Services Description / Analysis Type / Description Technology RUN TIME BHU & Start-Ups Other Academic Institution, R&D Labs, MSMEs Large Industrial Establishment

Deep Protein Analysis Protein Identification (Targeted for Single Protein) (60 min Run + Sample Preparation)

60 MIN 7500 15000 30000

Deep Protein Analysis [Total Protein Profiling- HRMS with database search - Protein Identification and Profiling (120 min Run + Sample Preparation)

120 MIN 9000 18000 36000

Single Metabolite Identification HRMS with database search - (Targeted for single molecule) Note- Up to 12 min Runtime

12 MIN 3100 6300 12600

Total Metabolite Profiling HRMS with database searchMetabolite Identification and Profiling Note - Up to 25 min Run time

25 MIN 6300 12600 25200

Deep Plasma Proteomics [Total Protein Profiling- HRMS with database search- Protein Identification and Profiling (120 min Run + Sample Preparation)]
Note- Top14 Abundant Protein Depletion will be done by facility side.

120 MIN 15000 30000 60000

Deep Phosphoprotein Enrichment analysis (Advance TiO2 + Fe-NTA Magnetic Phosphopeptide Enrichment Kit)     (60 min Run + Sample Preparation)
Note- Enrichment will be done by facility side.

60 MIN 16000 32000 64000

Deep Phosphoprotein Enrichment analysis (Advance TiO2 + Fe-NTA Magnetic Phosphopeptide Enrichment Kit) (60 min Run + Sample Preparation)
Note- Enrichment will be done by facility side.

60 MIN 20000 40000 80000

Deep Protein Analysis (After Fractionation- total 5 fractions) [Total Protein Profiling- HRMS with database search- Protein Identification and Profiling (300 min Run + Sample Preparation)]
Note- Fractionation will be done by facility side.

300 MIN 25000 50000 80000

Labeled Quantification (TMT / iTRAQ) (After Fractionation- total 5 fractions) [Total Protein Profiling- HRMS with database search- Protein Identification and Profiling (300 min Run + Sample Preparation)]
Note- Labeling will be done by customer side.

300 MIN 25000 50000 80000
Note :- 18% GST is applicable with user charges.

In Case of Deep Protein Analysis :-

For Quantitative analysis Rs. 1000/- per sample additional charges will be applicable.

In case of Single Metabolite Identification HRMS with database search and Total Metabolite Profiling HRMS with database searchMetabolite Identification and Profiling :-

  • If the run time exceeds the limit the charges will be in multiple accordingly.
  • These charges are for the both (positive + negative) modes.
  • For single (positive + negative) mode small molecules identification the charges will be reduced accordingly.
  • For Quantitative analysis of small molecule Rs. 1000/- per sample additional charges will be applicable.

Note – For External samples, GST will be applicable as per rule.


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